Taguchi optimization and scale up of xylanase from Bacillus licheniformis isolated from hot water geyser
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چکیده
منابع مشابه
Optimization of Fermentation Conditions for Cellulases Production by Bacillus licheniformis MVS1 and Bacillus sp. MVS3 Isolated from Indian Hot Spring
The aim of this work was to study the effect of some nutritional and environmental factors on the production of cellulases, in particular endoglucanase (CMCase) and exoglucanases (FPase) from Bacillus licheniformis MVS1 and Bacillus sp. MVS3 isolated from an Indian hot spring. The characterization study indicated that the optimum pH and temperature value was 6.5 to 7.0 and 50-55C, respectively....
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Aims and Background: Glutamate-specific endopeptidase (GSE, EC 3.4.21.19) is belonging to the serine protease family enzymes. A glutamate-specific endopeptidase has the ability to cleavage peptide bonds for the protein structure analysis, solid phase synthesis of peptides and preparation of nanotubes. The purpose of this investigation was to produce glutamate endopeptidase enzyme from the Bacil...
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In the present investigation, a gene encoding extracellular lipase was cloned from a Bacillus licheniformis. The recombinant protein containing His-tag was expressed as inclusion bodies in Esherichia coli BL21DE3 cells, using pET-23a as expression vector. Expressed protein purified from the inclusion bodies demonstrated ~22 kDa protein band on 12 % SDS-PAGE. It exhibited specific activity of 0....
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L-Asparaginase has potential as an anti-cancer drug and for prevention of acrylamide formation in fried and baked foods. Production of the enzyme by Bacillus licheniformis (RAM-8) was optimized by process engineering using a statistical modeling approach and a maximum yield of 32.26 IU/ml was achieved. The L-asparaginase exhibited glutaminase activity of only 0.8 IU/ml and would therefore be le...
متن کاملOptimization of Process Parameters and Scale-up of Xylanase Production from Bacillus Amyloliquifaciens Sh8 in a Stirred Tank Bioreactor
Bacillus amyloliquifaciens SH8 has been explored as a potential xylanolytic in-house producer for xylanase production in a bioreactor by optimizing process conditions. The scale-up of the fermentation process up to 3.5 L along with the optimization of physical parameters yielded a significantly increased xylanase yield – of 67.80 IU/mL, in 8 h of fermentation at an inoculum size of 10% with an ...
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ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2020
ISSN: 2090-5920
DOI: 10.1186/s43141-020-00084-0